Banshi Nath

3 Questions 5 Answers 0 Followers

Questions related from Banshi Nath

Why my IP injcetion drug for mice turn opaque after thawing?

Hi, I have prepared a drug solution that is dissolved in DMSO. We are giving IP injection to mice. The final stock concentration is 2mg/ml in 5% DMSO, 2% Tween-80 and 93% Saline. Before injection,...

19 November 2020 6,141 2 View

Why I am not getting any band in my Gradient PCR from cDNA?

I have been trying to standardize the annealing temperature for my new primer set but unfortunately, I am not getting any band in gel except primer dimer. The Tm for my primer is a little bit high...

23 May 2017 776 4 View

How can I improve fixing of Neuronal Cells for IHC?

I have differentiated the Neuronal cells. I used the PDL coated chamber slides for staining but these cells are vary loose attached with surface so I am getting lesser no of cells at the time of...

02 February 2015 3,166 8 View