Can the size of a protein differ in soluble and insoluble fractions of proteins of E.coli?

I have expressed a gene encoding 15 kDa protein in E. coli BL21RIPL cells. I dissolved the pellet in 1M tris, pH7.5, then sonicated , centrifuged. now the supernatant(soluble fraction) and the...

07 July 2015 3,674 8 View