Banita Kumari

2 Questions 4 Answers 0 Followers

Questions related from Banita Kumari

Can the size of a protein differ in soluble and insoluble fractions of proteins of E.coli?

I have expressed a gene encoding 15 kDa protein in E. coli BL21RIPL cells. I dissolved the pellet in 1M tris, pH7.5, then sonicated , centrifuged. now the supernatant(soluble fraction) and the...

09 July 2015 8,238 7 View

I am not getting MgCl2.6H2O. Can I use MgCl2.4H2O instead of it by keeping molarity of MgCl2 same?

How can MgCl2.4H2O be used in place of MgCl2.6H2O?

25 February 2015 5,766 4 View