Banadipa Nanda

2 Questions 5 Answers 0 Followers

Questions related from Banadipa Nanda

How do I store my lysed, non-purified sample, if I am unable to purify the protein of interest on the same day as bacterial cell lysis ?

I am purifying a viral protease of size 33 kda in E.coli BL-21 expression system. After cell lysis due to some unforeseen technical problems I was unable to purify my protein of interest on the...

22 September 2021 7,036 5 View

I am unable to express my His-tagged protein in E.coli BL-21(DE3) cells

I am trying to express and purify a viral cysteine-protease inserted in pET28a expression vector. The host is E.Coli BL-21 (DE3). I have done the transformation experiment two times and colony was...

01 January 1970 4,671 3 View