Bana Daghlas

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Questions related from Bana Daghlas

Help dialyzing protein in a 100mM cysteine-containing buffer?

Hello, I am trying to dialyze a protein I'm working with in a buffer containing 100 mM L-cysteine. From what I've read, L-cysteine should be dissolved in a low pH solvent, in an airtight...

02 July 2019 7,672 4 View

How to troubleshoot steady state kinetics runs in which higher substrate concentration is not leveling off as it should?

Trying to master steady-state kinetics using a wild-type version of an enzyme my lab works with (PGM-1) against various concentrations of G1P (25uM-800uM). Obviously the protein concentration is...

16 June 2018 6,350 2 View