Balamurugan Rangasamy

2 Questions 7 Answers 0 Followers

Questions related from Balamurugan Rangasamy

Does Pfu Polymerase make much difference in PCR amplification than Taq polymerase?

I have isolated Dengue virus RNA from cell culture sup and serotyped as DENV 1. now i am trying to clone NS2b-NS3 whole protein, but am not getting product amplified. in the reference article...

13 September 2019 8,008 4 View

Which cell line is apt for dengue virus palque assay? and CMC or agarose?

I have isolated dengue virus from infected patient serum and for titration i did plaque assay by using different conc. of vero cells in 12well as well as in 24 well plate, but i didnt get...

21 June 2018 509 7 View