Bakhtiyar Taghizada

2 Questions 2 Answers 0 Followers

Questions related from Bakhtiyar Taghizada

Western band pattern is not reproducible after probing for loading control of the same species?

Hello, I ran 50ug whole cell extracts of HEK293 on Western and probed for yH2AX mouse overnight 4C (Image 1). After developing I probed the same blot with tubulin mouse again overnight 4C (Image...

07 May 2019 3,564 5 View

BP reaction, I get colonies but not the right construct?

After BP reaction I get quite few normal sized colonies, which indicates that the reaction worked. However, when I do double restriction digestion on the miniprepd plasmid from transformed...

09 March 2018 3,957 1 View