Bahja Al Riyami

2 Questions 11 Answers 0 Followers

Questions related from Bahja Al Riyami

What can I do to improve my protein purification?

I am trying to purify a protein for crystallization purpose, so I need an ultra-pure product. I expressed the protein as MBP-GFP fusion with His tag (at the C-terminal). The purification scheme...

20 October 2015 9,210 12 View

Why am I getting two bands after gel filtration although the chromatogram shows one peak?

I purified a recombinant protein that is His tagged. The purification scheme was affinity chromatography followed by TEV protease digest (to get rid of the His tag) affinity chromatography again,...

09 November 2014 463 18 View