How to get rid of the free GFP signal in an Atg8-GFP tagged yeast saccharomyces cerevisiae?

I am working with yeast saccharomyces cerevisiae -BY4741- strain. I tagged the Atg8 with GFP in this background and whenever I am performing western blot I get a free GFP signal for my WT which...

16 November 2020 8,783 3 View