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two proteins(23 kDa, 66 kDa) shown same band intensity on sds page with coomasie stainining. Then i measured the concentrations of both proteins with nano drop at 280nm directly, 1. with out...
29 October 2015 3,879 7 View
absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm, does it mean the protein solution contain dimers/oligomers or does it contain nucleic acid contamination?
29 October 2015 303 2 View
Hello Different molecular weights proteins 23 kDa and 66 kDa looks similar band intensity on SDS-PAGE(coomasie staining) does it mean both proteins contain same concentration? When I measured...
22 October 2015 9,165 14 View