Azmi Zaki Althaf

6 Questions 2 Answers 0 Followers

Questions related from Azmi Zaki Althaf

How to exclude chloroplast and mithocondrial sequence from my 16s metagenomic data?

I did 16s metagenomic sequencing using Nanopore. Then I analyzed it with EPI2ME. I got a lot of Cyanobacteria. I assume that they are chloroplast not bacteria. My sample is root of Oryza sativa. I...

20 February 2025 6,876 2 View

What is the minimum concentration of DNA template for qPCR?

I will do qPCR from DNA genome. My DNA concentration is only 2-0.5 ng/uL. What is the minimum concentration of DNA template for qPCR? If need more DNA concentration, what should I do? Thank you...

07 July 2023 378 2 View

What method should I use in qPCR?

I want to know the Relative abundance of Antimicrobial Resistance Genes by qPCR (I Will compare the abundance of ARGs and the abundance of bacteria in my sample). I Will use ARGs primers and 16s...

11 June 2023 4,280 1 View

How to make detection primers of Antimicrobial Resistance Genes?

I wanna make detection primer of AMR that I'll be used to detect AMGs in soil in bacteria. How to make the primers?

09 June 2023 3,194 0 View

Why detection primers for soil has different results??

I made detection primers to detect Antimicrobial Resistance Genes in soil that I used in conventional PCR. But, my primers have different results in different soil samples (I extract DNA in the...

09 June 2023 1,673 2 View

Can I compare the XRF results and AAS results?

I have 15 samples. 10 samples I measured with XRF and others I used AAS. Can I analys both of them with one analysis or I should measure all of them with one device (XRF or AAS)? I want to know...

05 June 2023 9,076 5 View