What is the fastest way to know whether your gRNAs (CAS9) are capable of introducing the double-stranded break (DSB) in the desired location?

I am using CRISPR Cas9 to introduce a point mutation in a gene to get patient-like cells. I am applying a plasmid with cas9 and my gRNA inserted. I used a ssODN as a template for introducing the...

04 May 2022 4,576 7 View