Azaz Ahmad

4 Questions 12 Answers 0 Followers

Questions related from Azaz Ahmad

Problem with Western blot protein transfer

I have a problem with not properly transferring proteins on Nitrocellulose membrane. The transfer protein look fuzzy and all over the membrane as you can see in the picture. The protein marker was...

22 November 2013 6,662 16 View

I have been told to generate a standard curve on real-time qPCR. Does anyone have protocol or can roughly explain how it works?

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05 June 2013 1,034 19 View

Amount of sample load on gel during Western Blot?

I have some confusion about the amount of sample normally required to load on gel during Western blot. I will be loading 50ug of sample in total volume of 20ul (including loading buffer) ....

09 May 2013 6,984 18 View

What lysis buffer is best for extracting proteins from fresh mouse brain tissue for western blot?

I will be using RIPA lysis buffer for other cells as mouse brain tissue has a lot of fats so my worry is will RIPA buffer work best on brain tissue? Another thing is if someone has any idea which...

13 February 2013 4,944 4 View