Azam Safary

2 Questions 7 Answers 0 Followers

Questions related from Azam Safary

Why I don't have any colonies in my cloning procedure?

I have a problem in my cloning procedure. Restriction enzymes (NdeI, NotI)Vector (5.5 Kb)Insert (0.9 kb)After digestion I perform gel extraction for vector and insert. Then I do ligation in volume...

09 September 2014 2,408 14 View

Is there an efficient method for refolding and purification of GST fusion inclusion body?

I have a GST-fusion protein (95 kDa) that forms inclusion body in Ecoli BL21 expression system.I couldn't solve inclusion body formation with reducing temperature and IPTG concentration, hens I...

08 August 2014 3,297 6 View