Azam Jamshidian

2 Questions 1 Answers 0 Followers

Questions related from Azam Jamshidian

I will be performing flow cytometry assessment of cytokine-receptor expression on the surface of freezed PBMCs. Does anyone have any experience?

Dear researchers I'm going to do an assay for the expression levels of some cytokine and chemokine receptors on the surface of PBMCs. The PBMCs are stored in -80 0c until the experiments. does any...

23 February 2016 1,091 4 View

How much is the viability percentage needed for doing a surface staining of cells for flow cytometry?

Dear Researchers We have some PBMC samples isolated from patients and stored for about 1 month in -20 freezer with FBS and DMSO. Due to reduced cell viability, I wonder if there is any possibility...

03 November 2015 5,983 7 View