How can avoid FITC bleaching during the FACS/ Imaging? I need suggestion for fixing the cancer cell line for FACS?

My study, FACS and imaging of surface marker of Breast cancer cell lines, i used Ab conjugate with FITC. I used 4% PFA for 10 min for fixing . I have fitc bleaching most of the time. can I use 2%...

04 April 2016 7,182 3 View