Ayusmita Ray

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Questions related from Ayusmita Ray

Can anyone suggest me how to ensure proper revival for the strain B. braunii UTEX 572?

I have bought strain B. braunii UTEX572 for my work, however, I do not see any positive growth after reviving it in both Bold 3N liquid and agar media supplemented with 10g/L glucose. Is there any...

11 March 2024 4,129 0 View

How to make agar plates using the drug 5-fluoroindole as it is light sensitive and sparingly soluble in water?

I need some suggestions on working with the drug 5-fluoroindole. I am working on green microalgae and trying to make my strain tryptophan auxotroph. However, I am unable to make proper drug plates...

15 February 2024 963 1 View

How to remove antibiotic markers from the microalgal genome post CRISPR-RNP based knock-in?

When CRISPR-Cas9 RNP-based gene knock-in is performed in green microalgae, the donor cassette will contain the GOI and some selection marker genes. Post the gene integration how do I remove the...

05 February 2024 6,648 1 View

Glycerol stocks of microalgae?

What percentage of glycerol is suitable for storing microalgae in -80C for long term purposes?

24 May 2023 9,879 2 View

Why do we not store microalgal cultures as glycerol stocks?

Can anyone explain why exactly microalgal cultures are not stored as glycerol stocks? What disadvantage does it have or the logic behind not opting for it?

06 July 2022 1,650 0 View