Aysegul Kaymak

4 Questions 11 Answers 0 Followers

Questions related from Aysegul Kaymak

How do you count your migrated cells in transwell migration plates?

Dear all, I have been trying to optimize a transwell migration assay. However I have some difficulties regarding the counting part. I am doing crstal violet staining and cleaning the upper part...

30 August 2016 2,708 6 View

Problem with packaging of pLKO-tet-on plasmid with 3rd generation packaging system?

Hi all, I have been trying to produce viral particles containing pLKO-tet-on plasmid in HEK cells. I am using Addgene's 3rd generation packaging system (pMDLg/pRRE, pRSV-Rev, pMD2.G) I followed...

23 March 2016 6,960 3 View

Why didn't my ligation work?

Hello all, I cut my 13.3 kb plasmid with EcoRI and XhoI. I didn't perform phosphatase treatment and continued directly with gel purification step of the plasmid. I tried to ligate a 114 bp insert...

17 January 2016 9,348 9 View

What is the best inducible lentiviral shRNA for mouse stem cell line?

I am planning to make inducible sh cell line for E14 cells. I am going to order either TRIPZ plasmid or Addgene's Tet-pLKO-puro plasmid. I am wondering if anyone could give me some feedback about...

04 January 2016 4,707 3 View