Aysegul Gungor Aydin

3 Questions 5 Answers 0 Followers

Questions related from Aysegul Gungor Aydin

Does anyone have any suggestion for labelling the neurons in ROI after the recording?

Hi folks, I need to find a stain/tracer to label the neurons within the recording sites. I was thinking to use DiI, but I am not sure that it will be helpful cause it requires a longer time in...

01 June 2022 8,523 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

16 February 2021 9,866 6 View

How Can I find a reverse primer with good rating, if I need to amplify entire gene?

I need to amplify the KCNA1 gene. I could not design the reverse primer with GC content ~50%, G/C content between 40-60%, rating 100, and no secondary structure. Any help much appreciated!

26 May 2020 7,416 9 View