6 Questions 3 Answers 0 Followers
Questions related from Ayoub Bouhadada
Hello Dear, Im PhD student I was using Gibsson assembly, and I cloned my gene onto the Psl18 plasmid . The plasmid was right when I sent it to Sequencing after geting a positives colonies....
27 August 2024 2,635 1 View
Hi, Im lokkimg for marker confocal microscopy for the reteculum endoplasmic (plant or algae). If some one can help me or give me some reources ! Thank you in advance for your assistance Ayoub
04 July 2024 1,685 0 View
Hello¸ Im PhD Student in UQTR ( Canada), I have a crucial experience for my project thesis : I have a WB (western blot) membrane that I need to store for a long time for incubation with other...
26 February 2024 7,117 1 View
Hey dears, I'm getting ready to create a genetic construction where I'll tag my protein with three flags. I'm aware that I must place it in either the C or N terminal, but I'm not sure which...
18 July 2023 559 2 View
Hellow, I have a question about PCR clean up. I amplifie my gêne CDBA From vector, and I looding it in gel agarose. after that, I cut my gel bande and I did PCR clean up. The problem is when...
22 April 2022 5,229 3 View
Hello, I need to address my protein in the ER (endoplasmic reteculum), for this I need to construct a new plasmid: does the peptide signal sequence need to be added inside my protein sequence...
01 January 1970 4,621 3 View