Ayon Hassan

3 Questions 17 Answers 0 Followers

Questions related from Ayon Hassan

Does anyone have any recommendation for a good kit for total RNA isolation from small sample size?

I am looking for a kit that can yield good quality RNA from small sample size (around 10 000 - 15 000 cells). I have tried with Trizol, but so far have been unsuccessful in getting good enough RNA...

05 May 2019 9,969 10 View

Can anyone help me with thymidine double block?

I am trying to synchronize my cells at G1/S with thymidine double block, 2mM final conc., 18h each block, with 8h release in between. I collected cells at different time points (starting from...

09 September 2018 3,646 6 View

Do CRISPR/Cas9 monoallelic knockout clones eventually become biallelic knockout?

I have a conceptual question. It is a commonly observed phenomenon when using the CRISPR/Cas9 system that edited cells harbor monoallelic knockout. However, in cells stably expressing Cas9 and...

05 May 2018 2,827 8 View