Aykut Bostancı

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Questions related from Aykut Bostancı

When i did T7E1 assay, i did not observe any band on agarose gel electrophoresis. What could be reason for this?

I'm doing T7E1 experiment to check CRISPR-Cas9 transfection efficiency. Although i see my PCR products in the true site on the agarose gel, i do not see any band when i do T7E1. It is as if no...

30 November 2023 793 3 View

Can be used lithium perchlorate (LiClO4) as GSK3 beta inhibitor?

As known lithium chloride (LiCl) is inhibitor of GSK3 beta and which binds to GSK3 beta active site instead of Mg++. So, we found lithium perchlorate from another lab and can i see same effect of...

08 January 2021 6,239 2 View

How is the thermodynamic selection of microrna 3p and 5p ends by Argonaute?

Hi All, Our study is about miRNA function. In miRNAs biogenesis, argounate proteins provide 3p or 5p selection of miRNAs with thermodynamic stability. What is this ''Thermodynamic Stability''?...

11 May 2020 3,611 0 View

Why do i observe BFP and GFP on same reporter cell?

I am using 293T-BFP reporter cell. I am doing gene editing with Cas9. 293T-BFP cells have repair template plasmid and when cas9 cuts BFP site on template, BFP converts GFP by adding donor...

16 September 2017 5,659 2 View