Ayesha Lcwu

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Questions related from Ayesha Lcwu

Problem with the purification of Crispr 12a? optimze the purifivation using fplc or histagged? Westrn blot also donot show any result except band?

I am currently working on crispr12a with 6x histtaged protein... i am using extraction buffer with with nacl, hepes and protease inhibitor....and in purification iam using nacl, hepesand...

19 August 2024 8,557 5 View

I am having some problem regarding crispr protein?

I have inserted crispr vector in BL21 competent cell with ampicillin resistance and after induction with IPTG for protein ...no cas protein band was obtained using SDS page.. not even near to its...

28 February 2024 8,268 4 View