Why are white spots appearing on gel zymography, post-Commassie staining and destaining?

I have been running gel zymography (7.5% polyacrylamide co-polymerized with 1% gelatin) loaded with 250 µg of protein / well from colonic lysate with the hopes of visualizing MMP-2 and -9. I had a...

21 April 2023 8,196 5 View