Ayça Olcay

2 Questions 4 Answers 0 Followers

Questions related from Ayça Olcay

When to examine knockdown of target after transfection?

I transfected a cell line with plasmids having shRNA inserts. After transfection, when should I examine the knockdown efficiency? I would like to isolate total RNA and do qPCR for that. I would be...

13 July 2021 6,301 3 View

How to isolate RNA from primary neuron culture?

Currently, I have a problem to isolate total RNA from mouse primary cortical neuron culture. I am seeding neurons to poly-L-lysine coated coverslips and seeding 500k cells per coverslips in a...

17 January 2021 4,988 8 View