Ayan Modak

3 Questions 5 Answers 0 Followers

Questions related from Ayan Modak

Why there is inconsistent reading or no difference in virus treated/infected in FITC-Dextran permeability assay with HMEC cells?

I'm a working on vascular leakage caused by virus infection. For this, I’m trying to standardize the permeability assay with HMEC (5×104 cells/insert) using corning inserts (0.33 mm, 0.4 um) and...

06 June 2019 5,856 2 View

How to study the levels of Phosphorylated Tight junction proteins like ZO1 and Occludin?

Hi all, I want to check the expression of phophorylated ZO1 and occludin from cell-lysate. However I couldn't get any Antibody commercially for phospho-ZO1 or Phospho-occludin as available for...

03 March 2018 6,119 3 View

What are the basic needs to establish a viral cell culture lab?

We at our lab have established the serological and molecular diagnosis of various viruses. Now we are in a try to establish the viral culture facility at our lab. it would be really great if...

07 July 2017 3,602 3 View