Ayan Saha

3 Questions 2 Answers 0 Followers

Questions related from Ayan Saha

How can I isolate enough RNA from 20K to 70K leukaemia cells for bulk RNA sequencing?

My samples are currently in Trizol (-80C).

01 March 2018 3,767 1 View

Hi guys, How can I calculate Relative Fluorescence Intensity (RFI) with flowcytometry?

I am trying to see the expression level of a receptor protein. I used PE as my isotype control. In some articles they used the formula given below. RFI = 10 × (3.5 × mean channel of antigen...

23 September 2016 3,272 3 View

What is the reason behind keeping the incubators humidified and at 5% CO2 during cancer cell culture?

I want to know specific reason behind setting these conditions.

14 February 2015 5,340 4 View