Aya Youssef

2 Questions 1 Answers 0 Followers

Questions related from Aya Youssef

RNA purity of hippocampus?

I measured RNA purity using uv spectro but absorbance at 260 give me negative value -0.232 i use 5micro sample and add 495 water for injection (rnase free water) why this is happened???

01 October 2024 7,089 3 View

Realtime -PCR problem why TNF-alpha not give any ct values?

I have made real time pcr with B-ACTIN and TNF-ALPHA . It give ct values with B actin but not give any values with TNF-alpha. I use 25microlitre pcr 1.50 microlitre primer 3 microlitre cdna...

06 November 2022 7,708 2 View