Avizit Das

3 Questions 7 Answers 0 Followers

Questions related from Avizit Das

How to identify fungal secondary metabolite biosynthesis gene clusters, which have not yet been identified in any other fungal genome till date?

I am studying fungal whole genome sequence specifically (basidiomycetes) secondary metabolite biosynthesis gene clusters. Antismash and SMURF have identified several terpenes, PKS, NRPS clusters...

22 August 2017 2,658 1 View

Can anyone suggest me an intact fungal DNA isolation protocol for whole genome sequencing?

SDS, CTAB method already used. at the same time genejet DNA purification kit and combination of mannual and genejet DNA purification kit applied. But each and every time DNA were fragmentated and...

30 July 2016 6,811 4 View

How can I amplify a sequence using degenerate primers where I tried before but only found the primer sequences matched?

PCR conditions were optimized both positive and negative controls were used sharp band was extracted with reliable gel extraction kit contigs have only the primer sequences to match with targeted...

04 October 2015 9,674 8 View