Avinchal Manhas

2 Questions 2 Answers 0 Followers

Questions related from Avinchal Manhas

Why few number of cells are expressing transgene on application of Dox despite the cell line being generated from single cell cloning?

I've generated a Dox-inducible transgene overexpressing cell line after lentiviral transduction followed by single-cell cloning. The problem I am facing is that only 20-30% of the cells express...

15 May 2023 9,386 1 View

Do we need to run unstained sample everytime before FACS to purify a cell population expressing surface marker?

I have a rare cell population and I am doing FACS sorting using cell surface marker staining to purify one cell type. I later plan on to pool the RNA from the same cell type from different...

28 January 2023 7,920 3 View