Avinash Kumar

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Questions related from Avinash Kumar

When I use SYBR Green II nucleic acid gel stain (1:10000 in TBE buffer) with mIRNA, why fluorescence decrease with increasing the concentration?

when I use SYBR Green II nucleic acid gel stain (1:10000 in TBE buffer) with mIRNA, why fluorescence decrease with increasing the concentration of mIRNA? I tried many times but same. I select the...

20 September 2024 2,818 3 View

Q1: How can I improve hybridization and the process of hybridization?

Procedure: Streptavidin-magnetic bead suspension (50 µL) was transferred into a vial. The beads were washed twice by the binding/washing buffer and then re-suspended in 100µL of the buffer. A...

17 May 2024 936 0 View