Austin Macleod Peasley

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Questions related from Austin Macleod Peasley

Are there any tools available for primer design using a degenerate DNA template?

I'm currently trying to design a set of degenerate primers to amplify an identified conserved region among our target proteins, but I can't find any tool that accepts non-ATCGN degenerate...

10 October 2017 3,052 2 View

Is 4°C or 10°C a better holding temperature for a PCR thermocycler?

For the moment we're using 4°C as the holding temperature for our more valuable/sensistive samples, and for more forgiving experiments like generating amplicons from plasmids were using 10°C to...

05 May 2015 4,648 12 View

Are there any known issues with protein function for fusion proteins that have a protein of interest bound to the 3' end of GFP?

We're testing protein localization using plasmids that place our gene of interest both 5' and 3' of the GFP, and while the plasmid with the protein of interest 5' of the GFP has clear and specific...

03 March 2015 3,331 11 View