Austin Wang

2 Questions 2 Answers 0 Followers

Questions related from Austin Wang

Why are the reporter assay results different in different cell lines?

I used the pGL4.2 vector to construct my target promoter vector and co-transfected it with an independent beta-gal vector as an internal control. I also transfected two different transcription...

27 February 2025 7,315 0 View

Using exogenous plasmid for protein overexpression, only mRNA levels increased, but the protein was not expressed. Why?

I am currently conducting a protein overexpression experiment and encountered an issue. I amplified the cDNA of my target protein using primers with an HA-tag sequence (attached to the 5’ end) via...

24 December 2024 1,714 2 View