Austin Macleod Peasley

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Questions related from Austin Macleod Peasley

Are there any tools available for primer design using a degenerate DNA template?

I'm currently trying to design a set of degenerate primers to amplify an identified conserved region among our target proteins, but I can't find any tool that accepts non-ATCGN degenerate...

27 October 2017 5,877 2 View

Is 4°C or 10°C a better holding temperature for a PCR thermocycler?

For the moment we're using 4°C as the holding temperature for our more valuable/sensistive samples, and for more forgiving experiments like generating amplicons from plasmids were using 10°C to...

18 May 2015 9,603 12 View