What is the reason for aggregation of HEK cells upon membrane homogenization?

For radioligand binding assays, our lab, for years, has used the following protocol for preparation of membrane. 1) Centrifuge cells (HEK 293 and CHO-K1) at 12,000 g and 4 C for 10 min following...

09 May 2018 3,652 2 View