I am tracing Ca2+ in endothelial cells using Fura2 AM and a fluorospectrometer. So far I have been getting a baseline higher than normal. any ideas?

Hi, Can anybody help me troubleshoot Calcium tracing? I am new at this and not sure where to begin. My protocol involves incubating cells in Fura+HBSS+calcium at 1uM in 37C for 25 min and wash...

22 June 2018 8,346 7 View