Augusto Peluso

2 Questions 4 Answers 0 Followers

Questions related from Augusto Peluso

Has anyone experienced different intensity of ECL signal in a same membrane of western blotting?

I have noted that when I reveal some PVDF membrane using ECL, different parts of membrane show different intensity of signal. (i.e. stronger signal on borders than in the middle).

11 November 2015 5,240 4 View

How can I improve protein extraction from HAEC cells for western blotting?

I need perform western blot for HAEC cells but the total protein I am extracting is not enough for it. I am using 75 cm² flasks and, according with my experience, this amount of cells should be...

03 March 2015 6,217 3 View