How do I overcome autofluorescence in cell stained with Alexa-Fluor 488-labeled antibody and imaged under inverted fluorescent microscope?

I am staining cells for MAGE-C1 expression but I have experienced autofluorescence of these cells. The cells are fixed with methanol or 4% Paraformaldehyde or Ethanol. They are blocked with 5%BSA...

24 October 2017 8,843 7 View