Audrey Rivière

2 Questions 5 Answers 0 Followers

Questions related from Audrey Rivière

How does one determine efficiency of amplification curves obtained by Taqman assays?

I would like to use the efficiency-corrected relative quantification methodology (Pfaffl, 2007). Where the Ct values for each RT-qPCR reaction are first transformed into expression values using...

21 August 2014 6,744 6 View

What is the formula for calculation of molecular mass of an amplicon?

I have difficulties finding a formula for the calculation of the molecular mass (g/mol) of an amplicon (double stranded). On the internet and in literature I find different formulas giving...

22 April 2014 8,382 6 View