Audrey Kow

4 Questions 12 Answers 0 Followers

Questions related from Audrey Kow

What causes cells to lose their properties?

Been doing beta-hexosaminidase release assay but the results hasn't been promising. Firstly, there's no colour development in the supernatant and secondly the release has been below 10% for all...

09 July 2015 1,444 6 View

What is the highest/acceptable concentration of plant extract used for MTT screening?

Planning to do a MTT (cytotoxicity) screening of a plant extract. Is there an acceptable range of concentration? Is 100mg/ml acceptable?

23 April 2015 9,459 3 View

Why isn't the colour in the supernatant sample (without Triton-X) developing after adding in glycine buffer in my beta hexosamidase assay?

I'm doing beta hexosamidase to check for the release of histamine from my cells. However, the colour of the supernatant samples develops only after a few days. What could be the reasons? Could it...

15 April 2015 6,237 10 View

Griess Assay

My DMEM media and cells' supernatant turned slight yellow upon addition of freshly prepared Griess reagent (1% sulphanilamide + 0.1% NED in 2.5% phosphoric acid). Has anyone encountered such...

01 January 1970 1,361 2 View