Atul Sathe

3 Questions 2 Answers 0 Followers

Questions related from Atul Sathe

How to get the correct transcript out of alternatively spliced transcripts?

I'm trying to clone the CDS of a gene. This gene harbors 12 Exons and 11 Introns. Size of the CDS is about 4 kb. After amplifying the CDS, I have cloned it into a cloning vector. Sequencing result...

07 August 2017 3,546 10 View

How far the promoter is located from the start codon (ATG) in Eukaryote genome?

I want to amplify the promoter region for one cloned gene in rice. For this I'm going to amplify 2 kb region (from genomic DNA) before the start codon (ATG). I have a concern regarding the reverse...

10 April 2017 1,160 6 View

How to reduce or eliminate the non-specific amplification in PCR?

I'm using the gene specific primer for screening the plant DNA samples. At the specific annealing temp (55) a 500 bp amplicon is getting amplified but there are significant non-specific bands too....

03 June 2014 6,391 12 View