Atul Sathe

5 Questions 3 Answers 0 Followers

Questions related from Atul Sathe

How to get the correct transcript out of alternatively spliced transcripts?

I'm trying to clone the CDS of a gene. This gene harbors 12 Exons and 11 Introns. Size of the CDS is about 4 kb. After amplifying the CDS, I have cloned it into a cloning vector. Sequencing result...

07 August 2017 3,556 10 View

How far the promoter is located from the start codon (ATG) in Eukaryote genome?

I want to amplify the promoter region for one cloned gene in rice. For this I'm going to amplify 2 kb region (from genomic DNA) before the start codon (ATG). I have a concern regarding the reverse...

10 April 2017 1,171 6 View

How can we overcome seed borne bacterial contamination in cotton?

We are working on plant tissue culture in cotton. The present bacterial contamination is seed borne (Gram -ve, cocci, in chains). We have tried to overcome it with 0.1% HgCl2, 0.1% SDS as well as...

03 March 2015 4,845 8 View

How to reduce or eliminate the non-specific amplification in PCR?

I'm using the gene specific primer for screening the plant DNA samples. At the specific annealing temp (55) a 500 bp amplicon is getting amplified but there are significant non-specific bands too....

03 June 2014 6,398 12 View

What is meant by Chimera/Chimeric transformation?

In case of genetic transformation (either Agrobacterium mediated or by particle bombardment), we will get transformed plant cells which may be either stably transformed or chimeric transformed...

11 February 2014 6,458 8 View