8 Questions 31 Answers 0 Followers
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Hi, I am trying to isolate monocyte from PBMC and induce them to make macrophages and microglia. I used monocyte attachment media and then change after 2 hours to media required for macrophage...
09 September 2019 1,317 4 View
Hi, I am working with a lncRNA (uncharacterized ) which i found to be present within the intonic region of my gene in human. When i blast it with mouse genome i did not find any sequence...
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Hi I am trying to express my lncRNA in neural cells. As transfection efficiency will be low i am planing to use lentivirus as my delivery method. But to be sure can i add a GFP protein using IRES...
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Hi All, I am trying to access changes in B cell markers in our knockout mouse and wild type from blood. I try to gate lymphocytes, then single cell, followed by live dead and B220+ cells. When i...
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Hi All I am trying to find out Crispr editing signatures in my experimental condition. My question is if i add a reporter construct /plasmid from outside by transfection, will i be able to isolate...
05 May 2018 1,084 3 View
Hi , i tried many transfection reagent and Viafect worked best for me when i used in HELA cell (knockdown for some protein) as observed by reporter plasmid (5.4 kb). When i do my experiments i...
05 May 2018 8,763 7 View
Hi can someone help me analyse my data i have performed real time pcr for 2 sets on day 1 Average ct values are given target genecontrol 22.9,,,,, 24.0 treatment 24.6,,,,, 24.9 Gapdh control...
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Hi I want to do NGS for microRNA presents in my exosomal samples obtained from cell culture. I have isolated exosomes followed by total RNA extraction. However, when I checked the quality on the...
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