How can I prevent Jurkat cell loss after triton-X permeablisation?

Hi, I'm trying to stain for actin in Jurkat cells the staining steps involve: 1. fixing cells in a 96 V bottom plate with 4% PFA 2. centrifuging at 1800 rpm for 3 mins 3. resuspending in 0.1%...

19 February 2024 7,494 0 View