4 Questions 18 Answers 0 Followers
Questions related from Athanasios Bethanis
Hey guys, I have a PCR product that is 66bp long and want to visualise in native PAGE but the bands are not as clear as I imagined they would be. I am preparing 10% gels that I pre-run for 30mins...
04 April 2018 8,035 6 View
Hi, I need to prepare the following elution buffer: - 40mM Tris/HCL, pH 8 - 10mM EDTA - 3.5M Urea - 0.02% Tween I had previously made this buffer by calculating the mass of each ingredient so...
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Hey, I was wondering if someone has prepared linear polyacrylamide (LPA) themselves before? I wanted to use it in DNA precipitation but it was far too expensive to buy whereas we already have...
12 December 2017 1,405 4 View
Hi, I've run a 2% agarose gel with just a ladder (GeneRuler 50bp) and my PCR product to make sure that the amplification worked. The ladder is visible however the band of the PCR products looks...
11 November 2017 5,675 9 View
