How to prevent "aster like" aggregates seen under the fluorescent microscopy of the in-vitro polymerized microtubules?

Hi I am preparing Microtubules polymerized in-vitro and observe them under fluorescent microscopy. In addition to the Microtubules, I see aggregates which are very brights dots ("aster like"). I...

15 August 2018 7,581 0 View