Atefeh Ebrahimian

3 Questions 4 Answers 0 Followers

Questions related from Atefeh Ebrahimian

Why the cells die after MTT addition even with lower concentration of MTT?

I did the Vero cell cultivation at different cell seeding density for toxicity effect study, and in the last two sets of MTT assays, cell died after MTT addition (even 10 minutes after, I just...

12 July 2022 690 4 View

Does anybody have this experience to see some gel precipitation after FBS thawing?

I have some bottles of FBS (same lot number) , after its thawing I sometimes observe some gel precipitation (you can see in the attached image) which even after heat inactivation they do not...

12 October 2021 3,368 3 View

For LDH activity, is there any difference to add NADH, pyruvate and Tris separately in the well or prepare a mixture of them and then add?

Hi everybody, For LDH enzyme activity, I prepared to different series of buffers based on two protocols: 1-I prepared the solutions of 100 mM Tris, 3.6 mM Sodium pyruvate, 3.4 mM NADH...

23 October 2020 9,979 3 View