What alteration does a coomassie brilliant blue G250 or bromophenol blue in loading buffer of SDS PAGE gels show and whether it affects quantification

We have tried running gels with both CBB G250 and bromophenol blue in separate gels and a significant variation was observed in both the gels. Bromophenol blue showed a smeared band and CBB showed...

23 June 2017 1,584 7 View