Hai,can anyone suggest modification for the neutrophil Isolation using ficoll paque and dextran method ,since we are getting dead cells using this?

We are using the Ficoll -dextran method for the isolation.We are not getting the white pellet after the addition of RBC lysis buffer. Usually we used to keep for 3 min after the addition of RBC...

27 December 2018 9,542 4 View