Any experience with passaging of murine BMDM? Do you keep L929 in your medium after passaging cells?

Hi all, I isolated murine BMDM. I trypsinized them after 10 days in L9299 medium and seeded them in DMEM + glutamax, mem and 10% FCS. However after passaging my cultures are not very healthy. Do I...

01 December 2016 5,965 3 View