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Questions related from Astelia Au
I obtained two peaks during the affinity chrokatography which are next to each other. After I run SDS-PAGE, both peaks are my target protein. I am curious about the reason behind that, can anyone...
08 August 2019 5,867 3 View
I am wondering whether the PEG prepared from powder is suitable for crystallisation purpose. As I read from the product speciation of PEG produced by Hampton Research, it mentioned that it is...
07 July 2019 3,716 7 View
If there is any online software that can reduce the number of contigs or forming longer sequence in whole genome sequencing for mycobacteria species? Thank you very much!
06 June 2019 301 1 View
I have went through some of the publications about the overexpression of protein for NMR studies. They had mentioned that the minimal media and autoinduction media can be used for producing...
05 May 2019 1,319 4 View
I have incubated my protein with thrombin, and it has successfully cleaved the tag from my protein. I was thinking that the thrombin should have show in the SDS-PAGE as well, but I didn't see any...
03 March 2019 5,636 12 View
My plasmid size is ~6000bp, when I extracted the plasmid and run it in 1% agarose, it shows one band above 1kb, one band in between 1kb and 7000bp, and one band in between 5200bp - 4000bp. There...
11 November 2018 7,003 6 View
GROMOS is popular with their MD simulation function, besides they can perform energy calculation and energy minimization of model as well. May I know is the energy minimization and energy...
03 March 2018 9,835 1 View
From my alignment results, there is a consecutive sequence (around 32 amino acids) which is present in my target but not in my template. After I modeled my protein with MODELLER, that sequence...
01 January 2018 2,347 6 View
